ras activation assay kit for elisa Search Results


ras activity  (Cytoskeleton Inc)
95
Cytoskeleton Inc ras activity
Ras Activity, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ras+activation+assay+kit+for+elisa/Ras+G-LISA+GTPase+Activation+Assay+Kit/pm24356578-60-4-21
Average 95 stars, based on 1 article reviews
ras activity - by Bioz Stars, 2026-10
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ras activation assay kit  (Merck KGaA)
90
Merck KGaA ras activation assay kit
Transformation of porcine MSCs. ( a ) Overview of stepwise transformation of genetically modified porcine MSCs. ( b ) Left: Cre-mediated excision of transcriptional termination cassette from the KRAS LSL-G12D allele. Cell types as shown. PCR amplification products across the site of the LSL-BS cassette in KRAS intron 1. Predicted fragment sizes: wild-type KRAS 167 bp; non-recombined KRAS LSL-G12D 1486 bp; Cre-excised KRAS L-G12D 201 bp. Right: Cre-mediated excision of transcriptional termination cassette from both TP53 LSL-R167H alleles. Cell types as shown. PCR amplification products across the site of LSL-NEO cassette in TP53 intron 1. Predicted fragment sizes: wild-type TP53 198 bp; non-recombined TP53 LSL-R167H 1929 bp; Cre-excised TP53 L-R167H 254 bp. ( c ) <t>Ras</t> <t>activation</t> <t>assay</t> and p53 western blotting analysis. Cre-recombined MSC-PKC and MSC-PKCM cells show increased levels of active GTP-bound Ras proteins (21 kDa) as well as abundant levels of mutant p53-R167H proteins (46 kDa). ( d ) EdU (5-ethynyl-2'-deoxyuridine) incorporation during S phase. Data are consistent with the enhanced proliferative capacity of genetically modified derivatives relative to wild-type MSCs. P -values: MSC-PK=0.0727; MSC-PKC=0.0042; MSC-PKCM=0.0019. ( e ) Upper row: Loss of contact inhibition. MSC-PKCM cells form multi-layered foci when cultured at higher densities. Scale bars indicate 400 μm. Lower row: Anchorage-independent growth in soft agar. Wild-type MSCs grow as single cells in semi-solid medium, whereas MSC-PK, MSC-PKC and MSC-PKCM cells form three-dimensional colonies. Scale bars indicate 400 μm. ( f ) Upper: H&E-stained section of paucicellular nodule formed by injected MSC-PKC cells. The nodule consists mainly of matrigel with fat islands, capillary sprouts and isolated cells with slightly irregular nuclei. Scale bar indicates 500 μm. The arrow indicates an area of higher cellularity. Lower: Mesenchymal cells with slightly irregular nuclei embedded in a myxoid stroma are evident at higher magnification. Scale bar indicates 100 μm. ( g ) H&E-stained sections of MSC-PKCM derived tumour. This tumour is mostly highly cellular, but a matrix-rich area is still present, for example, as indicated by an asterisk. In the cellular areas, large tumour cells with pleomorphic nuclei are evident. A chronic inflammatory reaction is also present. Scale bar indicates 200 μm.
Ras Activation Assay Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ras+activation+assay+kit+for+elisa/ras+activation+elisa+assay+kit/pmc04815050-195-6-10
Average 90 stars, based on 1 article reviews
ras activation assay kit - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
human gap ras gtpase activating protein elisa kit  (Elabscience)
N/A
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human syngap1(synaptic ras gtpase-activating protein 1) elisa kit  (AMS Biotechnology)
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Human SYNGAP1(Synaptic Ras GTPase-activating protein 1) ELISA Kit
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Transformation of porcine MSCs. ( a ) Overview of stepwise transformation of genetically modified porcine MSCs. ( b ) Left: Cre-mediated excision of transcriptional termination cassette from the KRAS LSL-G12D allele. Cell types as shown. PCR amplification products across the site of the LSL-BS cassette in KRAS intron 1. Predicted fragment sizes: wild-type KRAS 167 bp; non-recombined KRAS LSL-G12D 1486 bp; Cre-excised KRAS L-G12D 201 bp. Right: Cre-mediated excision of transcriptional termination cassette from both TP53 LSL-R167H alleles. Cell types as shown. PCR amplification products across the site of LSL-NEO cassette in TP53 intron 1. Predicted fragment sizes: wild-type TP53 198 bp; non-recombined TP53 LSL-R167H 1929 bp; Cre-excised TP53 L-R167H 254 bp. ( c ) Ras activation assay and p53 western blotting analysis. Cre-recombined MSC-PKC and MSC-PKCM cells show increased levels of active GTP-bound Ras proteins (21 kDa) as well as abundant levels of mutant p53-R167H proteins (46 kDa). ( d ) EdU (5-ethynyl-2'-deoxyuridine) incorporation during S phase. Data are consistent with the enhanced proliferative capacity of genetically modified derivatives relative to wild-type MSCs. P -values: MSC-PK=0.0727; MSC-PKC=0.0042; MSC-PKCM=0.0019. ( e ) Upper row: Loss of contact inhibition. MSC-PKCM cells form multi-layered foci when cultured at higher densities. Scale bars indicate 400 μm. Lower row: Anchorage-independent growth in soft agar. Wild-type MSCs grow as single cells in semi-solid medium, whereas MSC-PK, MSC-PKC and MSC-PKCM cells form three-dimensional colonies. Scale bars indicate 400 μm. ( f ) Upper: H&E-stained section of paucicellular nodule formed by injected MSC-PKC cells. The nodule consists mainly of matrigel with fat islands, capillary sprouts and isolated cells with slightly irregular nuclei. Scale bar indicates 500 μm. The arrow indicates an area of higher cellularity. Lower: Mesenchymal cells with slightly irregular nuclei embedded in a myxoid stroma are evident at higher magnification. Scale bar indicates 100 μm. ( g ) H&E-stained sections of MSC-PKCM derived tumour. This tumour is mostly highly cellular, but a matrix-rich area is still present, for example, as indicated by an asterisk. In the cellular areas, large tumour cells with pleomorphic nuclei are evident. A chronic inflammatory reaction is also present. Scale bar indicates 200 μm.

Journal: Oncogenesis

Article Title: A porcine model of osteosarcoma

doi: 10.1038/oncsis.2016.19

Figure Lengend Snippet: Transformation of porcine MSCs. ( a ) Overview of stepwise transformation of genetically modified porcine MSCs. ( b ) Left: Cre-mediated excision of transcriptional termination cassette from the KRAS LSL-G12D allele. Cell types as shown. PCR amplification products across the site of the LSL-BS cassette in KRAS intron 1. Predicted fragment sizes: wild-type KRAS 167 bp; non-recombined KRAS LSL-G12D 1486 bp; Cre-excised KRAS L-G12D 201 bp. Right: Cre-mediated excision of transcriptional termination cassette from both TP53 LSL-R167H alleles. Cell types as shown. PCR amplification products across the site of LSL-NEO cassette in TP53 intron 1. Predicted fragment sizes: wild-type TP53 198 bp; non-recombined TP53 LSL-R167H 1929 bp; Cre-excised TP53 L-R167H 254 bp. ( c ) Ras activation assay and p53 western blotting analysis. Cre-recombined MSC-PKC and MSC-PKCM cells show increased levels of active GTP-bound Ras proteins (21 kDa) as well as abundant levels of mutant p53-R167H proteins (46 kDa). ( d ) EdU (5-ethynyl-2'-deoxyuridine) incorporation during S phase. Data are consistent with the enhanced proliferative capacity of genetically modified derivatives relative to wild-type MSCs. P -values: MSC-PK=0.0727; MSC-PKC=0.0042; MSC-PKCM=0.0019. ( e ) Upper row: Loss of contact inhibition. MSC-PKCM cells form multi-layered foci when cultured at higher densities. Scale bars indicate 400 μm. Lower row: Anchorage-independent growth in soft agar. Wild-type MSCs grow as single cells in semi-solid medium, whereas MSC-PK, MSC-PKC and MSC-PKCM cells form three-dimensional colonies. Scale bars indicate 400 μm. ( f ) Upper: H&E-stained section of paucicellular nodule formed by injected MSC-PKC cells. The nodule consists mainly of matrigel with fat islands, capillary sprouts and isolated cells with slightly irregular nuclei. Scale bar indicates 500 μm. The arrow indicates an area of higher cellularity. Lower: Mesenchymal cells with slightly irregular nuclei embedded in a myxoid stroma are evident at higher magnification. Scale bar indicates 100 μm. ( g ) H&E-stained sections of MSC-PKCM derived tumour. This tumour is mostly highly cellular, but a matrix-rich area is still present, for example, as indicated by an asterisk. In the cellular areas, large tumour cells with pleomorphic nuclei are evident. A chronic inflammatory reaction is also present. Scale bar indicates 200 μm.

Article Snippet: Ras activity was analysed using the Ras Activation Assay Kit (Merck, Millipore, Darmstadt, Germany).

Techniques: Transformation Assay, Genetically Modified, Amplification, Activation Assay, Western Blot, Mutagenesis, Inhibition, Cell Culture, Staining, Injection, Isolation, Derivative Assay